Nrg4^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Nrg4^{tm1b(EUCOMM)Hmgu}
• Name: neuregulin 4; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7764927
• Synonyms: NRG KO
• Gene: Nrg4 Location: Chr9:55127506-55190937 bp, - strand Genetic Position: Chr9, 29.87 cM, cytoband C
• Alliance: Nrg4^{tm1b(EUCOMM)Hmgu} page
• IMPC: Nrg4 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:357775
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 55147697 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 55150123. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:357775)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nrg4 Mutation: 31 strains or lines available

References

• Original: J:357775 Chen Z, et al., Neuregulin 4 mediates the metabolic benefits of mild cold exposure by promoting beige fat thermogenesis. JCI Insight. 2023 Nov 28;:e172957
• All: 1 reference(s)