Rad18^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rad18^{tm1c(EUCOMM)Wtsi}
• Name: RAD18 E3 ubiquitin protein ligase; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7763570
• Synonyms: Rad18;DelCre;FlpE, RDF
• Gene: Rad18 Location: Chr6:112596811-112673647 bp, - strand Genetic Position: Chr6, 52.42 cM, cytoband F
• Alliance: Rad18^{tm1c(EUCOMM)Wtsi} page
• IMPC: Rad18 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:346582
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt0 cassette was inserted at position 112670209 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 112670934. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:346582)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rad18 Mutation: 31 strains or lines available

References

• Original: J:346582 Kalweit K, et al., Role of Rad18 in B cell activation and lymphomagenesis. Sci Rep. 2024 Mar 25;14(1):7066
• All: 1 reference(s)