Rad18^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rad18^{tm1b(EUCOMM)Wtsi}
• Name: RAD18 E3 ubiquitin protein ligase; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:7763569
• Synonyms: Rad18;delCre, RD
• Gene: Rad18 Location: Chr6:112596811-112673647 bp, - strand Genetic Position: Chr6, 52.42 cM, cytoband F
• Alliance: Rad18^{tm1b(EUCOMM)Wtsi} page
• IMPC: Rad18 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:346582
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_gt0 cassette was inserted at position 112670209 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 112670934. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:346582)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rad18 Mutation: 31 strains or lines available

References

• Original: J:346582 Kalweit K, et al., Role of Rad18 in B cell activation and lymphomagenesis. Sci Rep. 2024 Mar 25;14(1):7066
• All: 1 reference(s)