Gt(ROSA)26Sor^tm5(CDX2)Ics
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm5(CDX2)Ics
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 5, Mouse Clinical Institute
• MGI ID: MGI:7751202
• Synonyms: GT(ROSA)26Sor^{tm1(flag-CDX2)Ics}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm5(CDX2)Ics page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:294291
• Parent Cell Line: S3 (ES Cell)
• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The following elements were inserted into the locus: a loxP site, STOP cassette (pgk1 promoter, neomycin resistance gene coding sequence, and pgk1 and 3x SV40 poly(A) signal sequences), a second loxP site and human CDX2 cDNA sequence with FLAG-tag sequence and bovine GH poly(A) signal sequences. The human cDNA will only be expressed after Cre-mediated deletion of the neo cassette. (J:294291)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

References

• Original: J:294291 Grall E, et al., Severe head dysgenesis resulting from imbalance between anterior and posterior ontogenetic programs. Cell Death Dis. 2019 Oct 24;10(11):812
• All: 1 reference(s)