P2rx4^tm1.1Ics
Targeted Allele Detail

Summary

• Symbol: P2rx4^tm1.1Ics
• Name: purinergic receptor P2X, ligand-gated ion channel 4; targeted mutation 1.1, Mouse Clinical Institute
• MGI ID: MGI:7751142
• Gene: P2rx4 Location: Chr5:122845607-122867801 bp, + strand Genetic Position: Chr5, 62.53 cM
• Alliance: P2rx4^tm1.1Ics page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:355451
• Parent Cell Line: S3 (ES Cell)
• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: A loxP site was inserted into intron 8 and 3X SV40 polyA signal sequences, an FRT site flanked neomycin resistance gene cassette, a second loxP site, followed by a duplicate of the 3' end of intron 8, cDNA sequence coding for exons 9-11 (up to the internalization motif in the 3' end of exon 11) plus in-frame mCherry reporter cDNA sequence was inserted into exon 12 downstream of the stop codon. The neo cassette was removed through subsequent Flp-mediated recombination. The allele expresses wild-type peptide and only after Cre-mediated deletion of wild-type exons 9-12 will it express the mutated peptide where the sequence from the internalization motif onward has been replace with the mCherry peptide. (J:355451)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any P2rx4 Mutation: 29 strains or lines available

References

• Original: J:355451 Bertin E, et al., Increased surface P2X4 receptor regulates anxiety and memory in P2X4 internalization-defective knock-in mice. Mol Psychiatry. 2021 Feb;26(2):629-644
• All: 1 reference(s)