Syt13^em1.1Heli
Endonuclease-mediated Allele Detail

Summary

• Symbol: Syt13^em1.1Heli
• Name: synaptotagmin XIII; endonuclease-mediated mutation 1.1, Heiko Lickert
• MGI ID: MGI:7751135
• Synonyms: Syt13-Venus, Syt13^VF
• Gene: Syt13 Location: Chr2:92745446-92786403 bp, + strand Genetic Position: Chr2, 51.37 cM, cytoband E1
• Alliance: Syt13^em1.1Heli page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:319939
• Parent Cell Line: IDG3.2 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: The fluorescent reporter gene Venus was inserted in-frame with exon 6 that has the translational stop codon removed via CRISPR/Cas9 technology. Additionally, an FRT-flanked PGK promoter-driven neomycin resistance gene was inserted downstream of the Venus reporter. The selection cassette was removed via flp-mediated recombination. (J:319939)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Syt13 Mutation: 21 strains or lines available

References

• Original: J:319939 Tarquis-Medina M, et al., Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas. Int J Mol Sci. 2021 Nov 20;22(22)
• All: 2 reference(s)