Gt(ROSA)26Sor^{tm2(CAG-mCherry/GFP*)Ganl}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(CAG-mCherry/GFP*)Ganl}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Ian Ganley
• MGI ID: MGI:7751124
• Synonyms: auto-QC
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(CAG-mCherry/GFP*)Ganl} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:259188
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector consists of (from 5' to 3') an FRT3 site, PGK neo polyA cassette, a CAG promoter, a Kozak sequence (GCCACC), the mCherry-GFP-Map1lc3b (mCherry-GFP-LC3) coding sequence, a human growth hormone polyA sequence (hGHpA), an additional polyA sequence and a FRT site. Recombination mediated cassette exchange (RMCE) is used to insert the CAG promoter and the open reading frame for the mCherry-GFP-Map1lc3b fusion protein into the Gt(ROSA)26Sor locus. (J:259188)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

References

• Original: J:259188 McWilliams TG, et al., Basal Mitophagy Occurs Independently of PINK1 in Mouse Tissues of High Metabolic Demand. Cell Metab. 2018 Feb 6;27(2):439-449.e5
• All: 3 reference(s)