Pbx4^em1Hncs
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pbx4^em1Hncs
• Name: pre B cell leukemia homeobox 4; endonuclease-mediated mutation 1, Chunsheng Han
• MGI ID: MGI:7738767
• Synonyms: PBX4-FLAG KI
• Gene: Pbx4 Location: Chr8:70285141-70324942 bp, + strand Genetic Position: Chr8, 33.92 cM, cytoband C
• Alliance: Pbx4^em1Hncs page

Mutation origin

• Strain of Origin: (C57BL/6J x DBA)F1

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag)
• Mutation: Insertion
• Mutation details: CRISPR/Cas9 genome editing technology was used to insert a 3FLAG coding sequence at the 3' end of the coding region of the gene. Western blot analysis using an anti-FLAG antibody demonstrated strong protein expression in the testis and a low-level expression in the bone marrow of homozygous mutant mice. (J:349843)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pbx4 Mutation: 24 strains or lines available

References

• Original: J:349843 Ning Y, et al., Transcription factor PBX4 regulates limb development and haematopoiesis in mice. Cell Prolif. 2024 May;57(5):e13580
• All: 1 reference(s)