Opn5^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Opn5^{tm1c(KOMP)Wtsi}
• Name: opsin 5; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7738660
• Synonyms: Opn5^fl
• Gene: Opn5 Location: Chr17:42867674-42922286 bp, - strand Genetic Position: Chr17, 19.65 cM, cytoband B3
• Alliance: Opn5^{tm1c(KOMP)Wtsi} page
• IMPC: Opn5 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:279052
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s). The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:279052)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Opn5 Mutation: 22 strains or lines available

References

• Original: J:279052 Nguyen MT, et al., An opsin 5-dopamine pathway mediates light-dependent vascular development in the eye. Nat Cell Biol. 2019 Apr;21(4):420-429
• All: 3 reference(s)