Galm^{tm2d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Galm^{tm2d(EUCOMM)Hmgu}
• Name: galactose mutarotase; targeted mutation 2d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7738544
• Gene: Galm Location: Chr17:80434886-80492461 bp, + strand Genetic Position: Chr17, 50.46 cM
• Alliance: Galm^{tm2d(EUCOMM)Hmgu} page
• IMPC: Galm gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 80444917 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39) exon 2. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) exon2 at position 80445851. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele with exon 2 floxed. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Galm Mutation: 21 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)