Sting1^em1.1Ciphe
Endonuclease-mediated Allele Detail

Summary

• Symbol: Sting1^em1.1Ciphe
• Name: stimulator of interferon response cGAMP interactor 1; targeted mutation 1.1, Centre d'ImmunoPhenomique
• MGI ID: MGI:7720866
• Synonyms: Sting1^tm1.1Ciphe, STING-OST^fl, Tmem173^tm1Ciphe
• Gene: Sting1 Location: Chr18:35866732-35873607 bp, - strand Genetic Position: Chr18, 19.23 cM, cytoband B3
• Alliance: Sting1^em1.1Ciphe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:354118
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Epitope tag)
• Mutation: Insertion
• Mutation details: A loxP site and an FRT-flanked neomycin resistance cassette were inserted in intron 2, 110 bp upstream of the start codon, a Twin-Strep-tag coding sequence (OST) was appended at the 5 end of the first coding exon (exon 3), and a loxP site was inserted in intron 3, 40 bp downstream of the 3 end of exon 3. Flp-mediated recombination removed the neomycin resistance cassette, leaving exon 3 floxed. The fusion of the OST to the protein can be used in affinity purification. (J:354118)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sting1 Mutation: 45 strains or lines available

References

• Original: J:354118 Jneid B, et al., Selective STING stimulation in dendritic cells primes antitumor T cell responses. Sci Immunol. 2023;8(79)
• All: 1 reference(s)