Piezo2^em1.1Ddg
Endonuclease-mediated Allele Detail

Summary

• Symbol: Piezo2^em1.1Ddg
• Name: piezo-type mechanosensitive ion channel component 2; endonuclease-mediated mutation 1.1, David D Ginty
• MGI ID: MGI:7719842
• Synonyms: Piezo2^smFP-FLAG
• Gene: Piezo2 Location: Chr18:63143284-63520254 bp, - strand Genetic Position: Chr18, 35.95 cM, cytoband D3
• Alliance: Piezo2^em1.1Ddg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:348146
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6J)F1

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: Using Crispr/cas9 system, a FLAG-tagged spaghetti monster fluorescent protein (smFP-FLAG) was fused to the 3' end of mouse Piezo2 exon 54 and followed by an FRT-flanked neomycin cassette. smFP-FLAG encodes 10 FLAG peptides and an intact green fluorescent protein (GFP) chromophore. After achieving successful germline transmission, the frt-NeoR-frt cassette was excised. (J:101977, J:348146)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Piezo2 Mutation: 124 strains or lines available

Notes

ES cell line = G1, established from a blastocyst created by crossing a 129S6 mouse with a C57BL/6J mouse.

References

• Original: J:348146 Handler A, et al., Three-dimensional reconstructions of mechanosensory end organs suggest a unifying mechanism underlying dynamic, light touch. Neuron. 2023 Sep 13
• All: 3 reference(s)