Vim^{tm2(flpo)Ggen}
Targeted Allele Detail

Summary

• Symbol: Vim^{tm2(flpo)Ggen}
• Name: vimentin; targeted mutation 2, Giannicola Genovese
• MGI ID: MGI:7718776
• Synonyms: Vim^{FLEX(SA-H2B-eGFP-T2a-FlpO-WPRE-pA)}, Vim^FlpO
• Gene: Vim Location: Chr2:13579122-13587637 bp, + strand Genetic Position: Chr2, 10.04 cM, cytoband A2
• Alliance: Vim^{tm2(flpo)Ggen} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:365589
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, Recombinase, Reporter)
• Mutation: Insertion
• Vim^{tm2(flpo)Ggen} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Vim (MGI:98932)

• Mutation details: The targeting vector was designed with (from 5'to 3') an inward facing lox2272 site upstream of exon 2 of the vimentin(Vim) gene, followed by exons 3 and 4, and an inverted cassette flanked with inward facing loxP sites. The inverted floxed cassette contains a polyadenylation sequence, a woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), a Flp-recombinase (FlpO), T2A sequence, enhanced green fluorescent protein (EGFP), a human HIST1H2BB gene [histone 1 H2bb], a splice acceptor site and a second lox2272 site in opposite orientation to the first, followed by an FRT-flanked neomycin cassette and a second inward facing loxP site all downstream of exon 4. (J:101977, J:365589)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Vim (mouse) Summary of all recombinase alleles driven by Vim. Alliance mouse Vim gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Vim Mutation: 40 strains or lines available

References

• Original: J:365589 Perelli L, et al., Evolutionary fingerprints of epithelial-to-mesenchymal transition. Nature. 2025 Apr;640(8060):1083-1092
• All: 2 reference(s)