Rab35^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Rab35^{tm1d(EUCOMM)Hmgu}
• Name: RAB35, member RAS oncogene family; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7718591
• Synonyms: Rab35 KO
• Gene: Rab35 Location: Chr5:115769953-115785217 bp, + strand Genetic Position: Chr5, 56.1 cM
• Alliance: Rab35^{tm1d(EUCOMM)Hmgu} page
• IMPC: Rab35 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:353484
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_gt1 cassette was inserted at position 115777657 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 115778534. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:353484)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rab35 Mutation: 19 strains or lines available

References

• Original: J:353484 Clearman KR, et al., Rab35 Is Required for Embryonic Development and Kidney and Ureter Homeostasis through Regulation of Epithelial Cell Junctions. J Am Soc Nephrol. 2024 Jun 1;35(6):719-732
• All: 1 reference(s)