Ncan^em2Erog
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ncan^em2Erog
• Name: neurocan; endonuclease-mediated mutation 2, Cagla Eroglu
• MGI ID: MGI:7716884
• Synonyms: Neurocan deltaELS
• Gene: Ncan Location: Chr8:70545735-70573494 bp, - strand Genetic Position: Chr8, 34.15 cM
• Alliance: Ncan^em2Erog page

Mutation origin

• Strain of Origin: B6SJLF1/J

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s))
• Mutation: Intragenic deletion
• Mutation details: Exons 9-14 of the mouse Ncan gene were excised using CRISPR/cas9 methodologies. The mutation removes the EGF-like, Lectin-like, and Sushi domains (ELS), leaving the rest of the gene unaltered. It expresses an intact NCAN N-terminal domain but lacks the expression of the ELS domain by genomic PCR, immunofluorescence, and western blot (J:348404)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ncan Mutation: 47 strains or lines available

References

• Original: J:348404 Irala D, et al., Astrocyte-secreted neurocan controls inhibitory synapse formation and function. Neuron. 2024 May 15;112(10):1657-1675.e10
• All: 1 reference(s)