Igh^em2Abtx
Endonuclease-mediated Allele Detail

Summary

• Symbol: Igh^em2Abtx
• Name: immunoglobulin heavy chain complex; endonuclease-mediated mutation 2, Dan Rohrer
• MGI ID: MGI:7715239
• Synonyms: mIgDJ_D KO
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: CMTI-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The allele has the mouse immunoglobulin heavy chain complex (Igh) region diversity (D) and joining (J) segments deleted. Specifically, CRISPR/Cas9 genome editing in 129S6/SvEvTac-derived CMTI-1 embryonic stem (ES) cells was used to remove a ~55 kb mouse Igh D-J segment and replace it with a PGK-neomycin resistance cassette. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 47 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)