Igh^{em1(IGH*)Abtx}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Igh^{em1(IGH*)Abtx}
• Name: immunoglobulin heavy chain complex; endonuclease-mediated mutation 1, Dan Rohrer
• MGI ID: MGI:7715238
• Synonyms: Fully human HC
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: CMTI-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence, Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Igh^{em1(IGH*)Abtx} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  human	IGH (3492)

• Mutation details: The allele has the mouse immunoglobulin heavy chain complex (Igh) region variable (V), diversity (D) and joining (J) segments deleted and replaced with a human IGH V-D-J sequence. Specifically, CRISPR/Cas9 genome editing in 129S6/SvEvTac-derived CMTI-1 embryonic stem (ES) cells was used in two separate steps to deliver into the mouse Igh locus a ~139 kb sequence containing an array of 41 IGH V segments plus a fully functional complement of 27 IGH D and 6 IGH J gene segments. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 41 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)