Igs7^{tm62.2(tetO-tdTomato)Hze}
Targeted Allele Detail

Summary

• Symbol: Igs7^{tm62.2(tetO-tdTomato)Hze}
• Name: intergenic site 7; targeted mutation 62.2, Hongkui Zeng
• MGI ID: MGI:7712761
• Synonyms: Ai63, Ai63(TIT-tdT)-D
• Gene: Igs7 Location: unknown Genetic Position: Chr9, Syntenic
• Alliance: Igs7^{tm62.2(tetO-tdTomato)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (RMCE-ready, Reporter)
• Mutation: Insertion
• Mutation details: The targeting constructed introduced by recombinase mediated cassette exchange at the existing site inserted anan FRT3 site, two tandem copies of the chicken beta-globin HS4 insulator element (to reduce expression of the reporter gene in the absence of transactivator protein), a modified Tet response element (TRE or (tetO); described in detail below), a loxP-flanked STOP cassette (with stop codons in all three reading frames linked to a synthetic pA-hGHpA-PGKpA unit), a sequence encoding the tdTomato fluorescent protein (a non-oligomerizing DsRed fluorescent protein variant with a 12 residue linker fusing two copies of the protein (tandem dimer)), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a bovine growth hormone polyA signal, two tandem copies of the chicken beta-globin HS4 insulator element, an AttB site, a PGK-5'hygro cassette, an RNA splice donor and a FRT5 site. PhiC31-mediated recombination removed the hygromycin cassette and replaced it with the recombined AttB/AttP site (AttL). Mating with cre recombinase mice removed the floxed stop cassette. The allele is therefore TIGRE-frt3-Ins-TRE-tdTomato-WPRE-bGHpA-Ins-AttL.
  The modified TRE promoter used in these transgenic mice is the Tet-responsive P^tight promoter. This P^tight promoter contains a modified Tet response element (TRE^mod), which consists of seven direct repeats of a 36-bp sequence each containing the 19-bp tet operator sequence (tetO). The TRE^mod is just upstream of a minimal human cytomegalovirus (CMV) promoter (P^minCMVDelta), which lacks the enhancer that is part of the complete CMV promoter. Consequently, P>tight< is silent in the absence of binding of TetR or rTetR to the tetO sequences. (J:101977, J:219930)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs7 Mutation: 76 strains or lines available

References

• Original: J:219930 Madisen L, et al., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron. 2015 Mar 4;85(5):942-58
• All: 2 reference(s)