H3f3a^em4Njab
Endonuclease-mediated Allele Detail

Summary

• Symbol: H3f3a^em4Njab
• Name: H3.3 histone A; endonuclease-mediated mutation 4, Nada Jabado
• MGI ID: MGI:7709721
• Synonyms: H3f3a^LGFPLG34R
• Gene: H3f3a Location: Chr1:180630125-180641127 bp, - strand Genetic Position: Chr1, 84.46 cM, cytoband D2.3
• Alliance: H3f3a^em4Njab page

Mutation origin

• Strain of Origin: (C57BL/6NCrl x C3H/HeNCrl)F2

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Reporter)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: CRISPR/Cas9 technology inserted a cassette consisting of a loxP-flanked adenovirus splice-acceptor (adSA), a mEGFP, and three polyA sequences, followed by the H3f3a coding sequence carrying a glycine to arginine mutation at amino acid 34 (p.G34R) into intron 1. GRCm39 has the GGG to AGG mutation resulting in the G to R substitution at amino acid 35. In the absence of cre recombinase, mEGFP is expressed. Upon cre-mediated recombination, the floxed mEGFP can be excised which allows for expression of the mutant H3f3a. (J:353083)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any H3f3a Mutation: 33 strains or lines available

References

• Original: J:353083 Khazaei S, et al., Single substitution in H3.3G34 alters DNMT3A recruitment to cause progressive neurodegeneration. Cell. 2023 Mar 16;186(6):1162-1178.e20
• All: 1 reference(s)