Gt(ROSA)26Sor^{em1(CAG-Map2k1*K57N,-GFP)Psmi}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1(CAG-Map2k1*K57N,-GFP)Psmi}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Patrick Smits
• MGI ID: MGI:7709531
• Synonyms: R26^{GT-Map2k1-GFP}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1(CAG-Map2k1*K57N,-GFP)Psmi} page

Mutation origin

• Strain of Origin: C57BL/6Hsd

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{em1(CAG-Map2k1*K57N,-GFP)Psmi} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Map2k1 (MGI:1346866)	activating p.K57N misense mutation

• Gt(ROSA)26Sor^{em1(CAG-Map2k1*K57N,-GFP)Psmi} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: A vector containing a slice acceptor site, a CAG promoter, a loxP flanked stop gene trap cassette, Map2k1 cDNA with an activating p.K57N missense mutation, an internal ribosome entry site (IRES), and GFP cDNA was inserted into intron 1 via CRISPR/cas9 technology. In the absence of cre-recombination, approximately 5% of all Map2k1 transcripts contain the mutant codon, indicating the gene trap stop is leaky at low levels. (J:352974)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

References

• Original: J:352974 Smits PJ, et al., Endothelial cell expression of mutant Map2k1 causes vascular malformations in mice. Angiogenesis. 2023 Feb;26(1):97-105
• All: 1 reference(s)