Slc5a7^em1Rbl
Endonuclease-mediated Allele Detail

Summary

• Symbol: Slc5a7^em1Rbl
• Name: solute carrier family 5 (choline transporter), member 7; endonuclease-mediated mutation 1, Randy D Blakely
• MGI ID: MGI:7709464
• Synonyms: CHT^Val
• Gene: Slc5a7 Location: Chr17:54580618-54606062 bp, - strand Genetic Position: Chr17, 28.23 cM, cytoband D
• Alliance: Slc5a7^em1Rbl page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/Cas9 technology generated an ATT to GTG change resulting in an isoleucine to valine substitution at amino acid 89 (p.I89V). In addition, a synonymous codon change in the adjacent codon, GGA to GGC, was also introduced to reduce chance of repeated CRISPR activity on the edited sequence. (J:352999)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc5a7 Mutation: 43 strains or lines available

References

• Original: J:352999 Donovan E, et al., Disrupted Choline Clearance and Sustained Acetylcholine Release In Vivo by a Common Choline Transporter Coding Variant Associated with Poor Attentional Control in Humans. J Neurosci. 2022 Apr 20;42(16):3426-3444
• All: 1 reference(s)