Kdm1a^tm1.1Ebg
Targeted Allele Detail

Summary

• Symbol: Kdm1a^tm1.1Ebg
• Name: lysine (K)-specific demethylase 1A; targeted mutation 1, Elena Battaglioli
• MGI ID: MGI:7709217
• Gene: Kdm1a Location: Chr4:136277851-136330034 bp, - strand Genetic Position: Chr4, 68.8 cM
• Alliance: Kdm1a^tm1.1Ebg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:232045
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutation: Intragenic deletion
• Mutation details: A floxed neomycin resistance cassette replaced exon 8a, which is required for the neurospecific isoform. RT-PCR confirmed the lack of neurospecific isoform transcript in the hippocampus. The floxed neo cassette was subsequently removed via cre-mediated recombination in the germline, leaving a single loxP site in place of exon 8a. (J:232045, J:235553)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kdm1a Mutation: 42 strains or lines available

References

• Original: J:232045 Rusconi F, et al., LSD1 modulates stress-evoked transcription of immediate early genes and emotional behavior. Proc Natl Acad Sci U S A. 2016 Mar 29;113(13):3651-6
• All: 2 reference(s)