Pgbd5^tm2.1Aken
Targeted Allele Detail

Summary

• Symbol: Pgbd5^tm2.1Aken
• Name: piggyBac transposable element derived 5; targeted mutation 2.1, Alex Kentsis
• MGI ID: MGI:7705048
• Gene: Pgbd5 Location: Chr8:125095788-125161230 bp, - strand Genetic Position: Chr8, 72.65 cM
• Alliance: Pgbd5^tm2.1Aken page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: iTL BF1 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector was designed to insert sequence for three FLAG tags (3xFlag), a hemagglutinin (HA) tag, a P2A self-cleaving peptide, followed by an enhanced green fluorescent protein (eGFP) and an FRT-flanked neomycin cassette upstream of the stop codon in exon 7 of the piggyBac transposable element derived 5 (Pgbd5) locus. Flp-recombinase removed the neomycin cassette. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pgbd5 Mutation: 35 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)