Gt(ROSA)26Sor^{em5(CAG-Tbx2,-Ikzf2)Zyliu}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em5(CAG-Tbx2,-Ikzf2)Zyliu}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 4, Zhiyong Liu
• MGI ID: MGI:7703104
• Synonyms: Rosa26-LSL-Tbx2-Ikzf2, Rosa26^Tbx2-Ikzf2
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em5(CAG-Tbx2,-Ikzf2)Zyliu} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Epitope tag, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{em5(CAG-Tbx2,-Ikzf2)Zyliu} expresses 2 genes
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ikzf2 (MGI:1342541)
  mouse	Tbx2 (MGI:98494)

• Mutation details: Using CRISPR/Cas9 technology, a cassette encoding a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG) followed by (from 5' to 3') a floxed STOP cassette and a 3xHA epitope C-terminal tagged T-box 2 (Tbx2) cDNA, a viral 2A oligopeptide (P2A) self-cleaving peptide, followed by a 3xV5 epitope C-terminal tagged IKAROS family zinc finger 2 (Ikzf2) cDNA, a viral 2A oligopeptide (T2A) self-cleaving peptidee and a EGFP sequence and a woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) fused to a polyadenylation signal were inserted into the Gt(ROSA)26Sor locus. (J:351380)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1083 strains or lines available

References

• Original: J:351380 Bi Z, et al., Revisiting the Potency of Tbx2 Expression in Transforming Outer Hair Cells into Inner Hair Cells at Multiple Ages In Vivo. J Neurosci. 2024 Jun 5;44(23)
• All: 1 reference(s)