Del(13Rr494-Macroh2a1)2Andg
Endonuclease-mediated Allele Detail

Summary

• Symbol: Del(13Rr494-Macroh2a1)2Andg
• Name: deletion, Chr 13, Guillaume Andrey 2
• MGI ID: MGI:7666514
• Synonyms: Pitx1^del3
• Gene: Del(13Rr494-Macroh2a1)2Andg Location: unknown Genetic Position: Chr13, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:268374
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Endonuclease-mediated (Modified regulatory region, Null/knockout)
• Mutations: Intergenic deletion, Intragenic deletion
• Del(13Rr494-Macroh2a1)2Andg involves 2 genes/genome features (Rr494, Macroh2a1) View all
• Mutation details: A section of genomic sequence, containing Pitx1 enhancer RA4 and most of the Macroh2a1 gene (with only a small part of the 5' end remaining), was deleted using sgRNAs (equivalent to TGCTAGGGACCTTACTGTAG and TAATGTATTAGAGGGGCGGC) with CRISPR/Cas9 technology. (J:268374)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Del(13Rr494-Macroh2a1)2Andg Mutation: 0 strains or lines available

References

• Original: J:268374 Kragesteen BK, et al., Dynamic 3D chromatin architecture contributes to enhancer specificity and limb morphogenesis. Nat Genet. 2018 Oct;50(10):1463-1473
• All: 1 reference(s)