Camk2b^{em1(CAMK2B)Rkuhn}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Camk2b^{em1(CAMK2B)Rkuhn}
• Name: calcium/calmodulin-dependent protein kinase II, beta; endonuclease-mediated mutation 1, Ralf Kuhn
• MGI ID: MGI:7666156
• Gene: Camk2b Location: Chr11:5919644-6016362 bp, - strand Genetic Position: Chr11, 3.89 cM
• Alliance: Camk2b^{em1(CAMK2B)Rkuhn} page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Sequence (100 bp) containing intronic splicing regulation sequence (in 3' end of ENSMUST00000002817 intron 13, ENSMUST00000019133 intron 14, or "global" intron 15 when taking all possible coding exons into account) was removed and replaced with the human equivalent using an sgRNA and a human minigene template with CRISPR/Cas9 technology. The sequence contains species-specific cis-regulatory sequences that determine the branch point strength in the pre-mRNA, which determines whether or not the downstream exon is spliced-in or skipped. (J:337559)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Camk2b Mutation: 93 strains or lines available

References

• Original: J:337559 Franz A, et al., Branch point strength controls species-specific CAMK2B alternative splicing and regulates LTP. Life Sci Alliance. 2023 Mar;6(3)
• All: 1 reference(s)