Cd74^em1Hrtn
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cd74^em1Hrtn
• Name: CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); endonuclease-mediated mutation 1, Cole Harrington
• MGI ID: MGI:7666006
• Synonyms: Cd74^tdT
• Gene: Cd74 Location: Chr18:60936921-60945724 bp, + strand Genetic Position: Chr18, 34.41 cM
• Alliance: Cd74^em1Hrtn page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: Using CRISPR/Cas9 technology, a P2A self-cleaving peptide sequence, a red fluorescent protein (tdTomato) sequence, and woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) followed by a polyadenylation signal were inserted to replace the stop codon of the endogenous CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) (Cd74) gene. (J:351008)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cd74 Mutation: 37 strains or lines available

References

• Original: J:351008 Harrington EP, et al., MHC class I and MHC class II reporter mice enable analysis of immune oligodendroglia in mouse models of multiple sclerosis. Elife. 2023 Apr 14;12
• All: 1 reference(s)