Gt(ROSA)26Sor^{em2(CAG-cas12a*)Sidi}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em2(CAG-cas12a*)Sidi}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 2, Sidi Chen
• MGI ID: MGI:7662593
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em2(CAG-cas12a*)Sidi} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: Using CRISPR/Cas9 technology, the intron 1 of the mouse Gt(ROSA)26Sor locus was targeted to insert LoxP-3x PolyA (Stop)-LoxP (LSL)-NLS-LbCas12a-NLS-HA-P2A-EGFP cassette. LbCas12a is a Lachnospiraceae bacterium (Lb)-derived variant of Cas12a/Cpf1. The LbCas12a gene was flanked by nuclear localization signals (NLSs) to attain better editing efficiency from higher nuclear translocation rates of LbCas12a. A hemagglutinin affinity tag (3xHA) was fused to the C-terminus of LbCas12a, then followed by a 2A self-cleavage peptide (P2A) and subsequent fluorescent marker, enhanced GFP (EGFP). (J:101977, J:363522)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1042 strains or lines available

References

• Original: J:363522 Tang K, et al., Cas12a-knock-in mice for multiplexed genome editing, disease modelling and immune-cell engineering. Nat Biomed Eng. 2025 Mar 20
• All: 2 reference(s)