Sema6a^tm2.1Alk
Targeted Allele Detail

Summary

• Symbol: Sema6a^tm2.1Alk
• Name: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A; targeted mutation 2.1, Alex L Kolodkin
• MGI ID: MGI:7661008
• Gene: Sema6a Location: Chr18:47378321-47504267 bp, - strand Genetic Position: Chr18, 25.08 cM, cytoband C
• Alliance: Sema6a^tm2.1Alk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:343285
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6J)F1

Mutation description

• Allele Type: Targeted (Epitope tag, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting construct inserted an hemagglutinin (HA) tag is knocked in downstream of the signal peptide in exon 2 at the N-terminus of the extracellular domain and floxed exon 3. The frt-flanked neo cassette was removed by crossing the FLP deleter line. Cre-mediated recombination removed exon 3. (J:101977, J:343285, J:367089)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sema6a Mutation: 84 strains or lines available

References

• Original: J:343285 Lettieri A, et al., SEMA6A drives GnRH neuron-dependent puberty onset by tuning median eminence vascular permeability. Nat Commun. 2023 Dec 7;14(1):8097
• All: 3 reference(s)