n-TUtca2^tm1.1Mym
Targeted Allele Detail

Summary

• Symbol: n-TUtca2^tm1.1Mym
• Name: nuclear encoded tRNA selenocysteine 2 (anticodon TCA); targeted mutation 1.1, Masayuki Yamamoto
• MGI ID: MGI:7660538
• Synonyms: Trsp^R
• Gene: n-TUtca2 Location: Chr7:19035169-19035255 bp, + strand Genetic Position: Chr7, 9.56 cM
• Alliance: n-TUtca2^tm1.1Mym page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:97632
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (No functional change)
• Mutation: Insertion
• Mutation details: A floxed neo-thymidine kinase cassette was inserted into the locus between the distal sequence element and proximal sequence element. RNase protection assay demonstrated that this disruption has a variable impact upon transcript levels in a tissue-ependent manner. Cre-mediated recombination removed the neomycin cassette to generate a rescue the allele. (J:97632)

Expression

In Mice Carrying this Mutation:

20 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any n-TUtca2 Mutation: 2 strains or lines available

References

• Original: J:97632 Kelly VP, et al., The Distal Sequence Element of the Selenocysteine tRNA Gene Is a Tissue-Dependent Enhancer Essential for Mouse Embryogenesis. Mol Cell Biol. 2005 May;25(9):3658-69
• All: 1 reference(s)