Sar1b^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sar1b^{tm1d(EUCOMM)Wtsi}
• Name: secretion associated Ras related GTPase 1B; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7658908
• Gene: Sar1b Location: Chr11:51654514-51682752 bp, + strand Genetic Position: Chr11, 31.41 cM
• Alliance: Sar1b^{tm1d(EUCOMM)Wtsi} page
• IMPC: Sar1b gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:349757
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_gt1 cassette was inserted at position 51678369 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 51679470. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:349757)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sar1b Mutation: 20 strains or lines available

References

• Original: J:349757 Tang VT, et al., Functional overlap between the mammalian Sar1a and Sar1b paralogs in vivo. Proc Natl Acad Sci U S A. 2024 May 7;121(19):e2322164121
• All: 1 reference(s)