Gt(ROSA)26Sor^{em1(CAG-LOC4337827*)Ahol}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1(CAG-LOC4337827*)Ahol}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Andrew Holland
• MGI ID: MGI:7657342
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1(CAG-LOC4337827*)Ahol} page

Mutation origin

• Strain of Origin: (C57BL/6 x SJL)F2

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Epitope tag, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: Using CRISPR/Cas9 technology, single guide RNA [5'-actccagtctttctagaaga-3'] was used to introduce a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP cassette including a puromycin cassette, the codon-optimized Oryza sativa F-box transport inhibitor response 1 (TIR1) sequence harboring the F74G modification and a c-myc (Myc) epitope tag, into intron 1 of the Gt(ROSA)26Sor locus on chromosome 6. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)