Gars1^em2Gebai
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gars1^em2Gebai
• Name: glycyl-tRNA synthetase 1; endonuclease-mediated mutation 2, Ge Bai
• MGI ID: MGI:7645901
• Synonyms: Gars^P234KY-V5
• Gene: Gars1 Location: Chr6:55014992-55056485 bp, + strand Genetic Position: Chr6, 27.29 cM
• Alliance: Gars1^em2Gebai page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Using oocytes that carry the Gars1^em2Gebai allele (where sequence for the V5 epitope tag was added in-frame to the 3' end of the coding region), proline codon 278 (CCT) in exon 7 was replaced with lysine + tyrosine (AAATAT) (p.P278delinsKY) using sgRNAs and ssODN templates with CRISPR/Cas9 technology. (J:347500)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gars1 Mutation: 43 strains or lines available

References

• Original: J:347500 Cui Q, et al., Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules. Cell. 2023 Feb 16;186(4):803-820.e25
• All: 1 reference(s)