Nfe2l2^tm2Mym
Targeted Allele Detail

Summary

• Symbol: Nfe2l2^tm2Mym
• Name: nuclear factor, erythroid derived 2, like 2; targeted mutation 2, Masayuki Yamamoto
• MGI ID: MGI:7645688
• Synonyms: Nrf2^L30F
• Gene: Nfe2l2 Location: Chr2:75505857-75534985 bp, - strand Genetic Position: Chr2, 44.75 cM
• Alliance: Nfe2l2^tm2Mym page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:347771
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Null/knockout, Reporter)
• Mutations: Insertion, Single point mutation
• Mutation details: Leucine codon 30 (CTT) in exon 2 was changed to phenylalanine (TTT) (p.L30F) and a loxP site flanked beta-galactosidase and neomycin resistance fusion gene cassette was inserted into intron 1. The mutation is the equivalent of the same human mutation associated with esophageal squamous cell carcinoma (ESCC). This allele is a reporter knockout allele and only after Cre-mediated removal of the beta-geo cassette will it express the mutated transcript and peptide. (J:347771)

Tumor Data

List all tumor models in MMHCdb carrying Nfe2l2^tm2Mym

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nfe2l2 Mutation: 94 strains or lines available

References

• Original: J:347771 Takahashi J, et al., Differential squamous cell fates elicited by NRF2 gain of function versus KEAP1 loss of function. Cell Rep. 2024 Apr 23;43(4):114104
• All: 1 reference(s)