Nlrp3^em1Helin
Endonuclease-mediated Allele Detail

Summary

• Symbol: Nlrp3^em1Helin
• Name: NLR family, pyrin domain containing 3; endonuclease-mediated mutation 1, Hening Lin
• MGI ID: MGI:7645401
• Synonyms: NLRP3 C126A
• Gene: Nlrp3 Location: Chr11:59432395-59457781 bp, + strand Genetic Position: Chr11, 37.73 cM, cytoband B1.3
• Alliance: Nlrp3^em1Helin page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Cysteine codon 126 (TGT) in exon 3 was changed to alanine (GCA) (p.C126A) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation blocks palmitoylation of the affected residue in the encoded peptide. (J:347882)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nlrp3 Mutation: 69 strains or lines available

References

• Original: J:347882 Yu T, et al., NLRP3 Cys126 palmitoylation by ZDHHC7 promotes inflammasome activation. Cell Rep. 2024 Apr 23;43(4):114070
• All: 1 reference(s)