Pcna^em1Gpc
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pcna^em1Gpc
• Name: proliferating cell nuclear antigen; endonuclease-mediated mutation 1, Gerry P Crossan
• MGI ID: MGI:7643759
• Synonyms: Pcna^K164R
• Gene: Pcna Location: Chr2:132091206-132095100 bp, - strand Genetic Position: Chr2, 64.15 cM
• Alliance: Pcna^em1Gpc page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Single point mutation
• Mutation details: Lysine codon 164 (AAG) in exon 4 was changed to arginine (AGG) (p.K164R) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation blocks monoubiquitination at the affected residue in the encoded peptide. (J:348075)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pcna Mutation: 17 strains or lines available

References

• Original: J:348075 Shah P, et al., Primordial germ cell DNA demethylation and development require DNA translesion synthesis. Nat Commun. 2024 May 3;15(1):3734
• All: 1 reference(s)