Zdhhc3^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Zdhhc3^{tm1c(EUCOMM)Hmgu}
• Name: zinc finger, DHHC domain containing 3; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7643400
• Gene: Zdhhc3 Location: Chr9:122895224-122942269 bp, - strand Genetic Position: Chr9, 73.66 cM, cytoband F4
• Alliance: Zdhhc3^{tm1c(EUCOMM)Hmgu} page
• IMPC: Zdhhc3 gene page

Mutation origin

• Mutant Cell Lines: HEPD0531_1_B09, HEPD0531_1_B12
• Germline Transmission: Earliest citation of germline transmission: J:348652
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 122917808 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 122918641. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:348652)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Zdhhc3 Mutation: 36 strains or lines available

References

• Original: J:348652 Baldwin TA, et al., Palmitoylation-dependent regulation of cardiomyocyte Rac1 signaling activity and minor effects on cardiac hypertrophy. J Biol Chem. 2023 Nov 3;299(12):105426
• All: 1 reference(s)