Otud3^tm1.2Nju
Targeted Allele Detail

Summary

• Symbol: Otud3^tm1.2Nju
• Name: OTU domain containing 3; targeted mutation 1.2, Model Animal Research Center of Nanjing University
• MGI ID: MGI:7641520
• Gene: Otud3 Location: Chr4:138622690-138641256 bp, - strand Genetic Position: Chr4, 70.57 cM, cytoband D3
• Alliance: Otud3^tm1.2Nju page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:282134
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2 and an FRT-flanked neomycin selection cassette and second loxP site were inserted downstream of exon 2. The selection cassette was removed via flp-mediated recombination. Exon 2 was deleted via cre-mediated recombination in the germline. The frame-shift caused by lack of exon 2 produces stop codons in exon 3. Western blot analysis confirmed absence of protein. (J:282134)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Otud3 Mutation: 24 strains or lines available

References

• Original: J:282134 Du T, et al., The deubiquitylase OTUD3 stabilizes GRP78 and promotes lung tumorigenesis. Nat Commun. 2019 Jul 2;10(1):2914
• All: 3 reference(s)