Tet1^em2Msb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tet1^em2Msb
• Name: tet methylcytosine dioxygenase 1; endonuclease-mediated mutation 2, Marisa S Bartolomei
• MGI ID: MGI:7641518
• Synonyms: Tet1^{H1654Y,D1656A}, Tet1^HxD
• Gene: Tet1 Location: Chr10:62640349-62723242 bp, - strand Genetic Position: Chr10, 32.48 cM
• Alliance: Tet1^em2Msb page

Mutation origin

• Strain of Origin: C57BL/6J x DBA/2J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Histidine codon 1652 (CAC) in exon 11 was changed to tyrosine (TAC) (p.H1652Y) and aspartic acid codon 1654 (GAC) was changed to alanine (GCC) (p.D1654A) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutations render the encoded peptide catalytically inactive. (J:348315)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tet1 Mutation: 147 strains or lines available

References

• Original: J:348315 Prasasya RD, et al., Iterative oxidation by TET1 is required for reprogramming of imprinting control regions and patterning of mouse sperm hypomethylated regions. Dev Cell. 2024 Apr 22;59(8):1010-1027.e8
• All: 1 reference(s)