Tet1^em1Msb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tet1^em1Msb
• Name: tet methylcytosine dioxygenase 1; endonuclease-mediated mutation 1, Marisa S Bartolomei
• MGI ID: MGI:7641517
• Synonyms: Tet1^T1642V, Tet1^V
• Gene: Tet1 Location: Chr10:62640349-62723242 bp, - strand Genetic Position: Chr10, 32.48 cM
• Alliance: Tet1^em1Msb page

Mutation origin

• Strain of Origin: C57BL/6J x DBA/2J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Threonine codon 1642 (ACC) in exon 11 was changed to valine (GTC) (p.T1642V) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation changes the catalytic activity of the encoded peptide so that it oxidizes 5mC to 5-hydroxymethyl-cytosine (5hmC) but no further. (J:348315)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tet1 Mutation: 147 strains or lines available

References

• Original: J:348315 Prasasya RD, et al., Iterative oxidation by TET1 is required for reprogramming of imprinting control regions and patterning of mouse sperm hypomethylated regions. Dev Cell. 2024 Apr 22;59(8):1010-1027.e8
• All: 1 reference(s)