Kif3a^em1Zfu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Kif3a^em1Zfu
• Name: kinesin family member 3A; endonuclease-mediated mutation 1, Zheng Fu
• MGI ID: MGI:7638779
• Synonyms: mKif3a^T674A
• Gene: Kif3a Location: Chr11:53458206-53492794 bp, + strand Genetic Position: Chr11, 31.97 cM, cytoband A5-B1
• Alliance: Kif3a^em1Zfu page

Mutation origin

• Strain of Origin: (C57BL/6 x SJL)F1

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Threonine codon 674 (ACC) in exon 17 was changed to alanine (GCA) (p.T674A) using crRNA, tracrRNA and an ssODN template with CRISPR/Cas9 technology. The mutation prevents phosphorylation of the affected residue in the encoded peptide. (J:302180)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kif3a Mutation: 31 strains or lines available

References

• Original: J:302180 Gailey CD, et al., Phosphosite T674A mutation in kinesin family member 3A fails to reproduce tissue and ciliary defects characteristic of CILK1 loss of function. Dev Dyn. 2021 Feb;250(2):263-273
• All: 1 reference(s)