Alms1^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Alms1^{tm1d(EUCOMM)Hmgu}
• Name: ALMS1, centrosome and basal body associated; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7628377
• Synonyms: Alms1 KO
• Gene: Alms1 Location: Chr6:85564513-85679735 bp, + strand Genetic Position: Chr6, 37.48 cM
• Alliance: Alms1^{tm1d(EUCOMM)Hmgu} page
• IMPC: Alms1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:347860
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 85591742 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 85592657. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:347860)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Alms1 Mutation: 151 strains or lines available

References

• Original: J:347860 McKay EJ, et al., Mesenchymal-specific Alms1 knockout in mice recapitulates metabolic features of Alstrom syndrome. Mol Metab. 2024 Apr 6;84:101933
• All: 2 reference(s)