Arid5b^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Arid5b^{tm1d(KOMP)Wtsi}
• Name: AT-rich interaction domain 5B; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7628241
• Gene: Arid5b Location: Chr10:67928350-68114570 bp, - strand Genetic Position: Chr10, 35.14 cM, cytoband B5.1
• Alliance: Arid5b^{tm1d(KOMP)Wtsi} page
• IMPC: Arid5b gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:347804
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 67964493 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 67965285. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:347804)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Arid5b Mutation: 141 strains or lines available

References

• Original: J:347804 Chalise JP, et al., ARID5B regulates fatty acid metabolism and proliferation at the Pre-B cell stage during B cell development. Front Immunol. 2023;14:1170475
• All: 1 reference(s)