Gramd1c^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Gramd1c^{tm1c(KOMP)Wtsi}
• Name: GRAM domain containing 1C; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7626290
• Synonyms: Gramd1c^fl
• Gene: Gramd1c Location: Chr16:43800713-43883708 bp, - strand Genetic Position: Chr16, 28.44 cM
• Alliance: Gramd1c^{tm1c(KOMP)Wtsi} page
• IMPC: Gramd1c gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:342939
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 43812106 of Chromosome 16 upstream of the exon 11 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the exon 11 at position 43812967. Exon 11 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:342939)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gramd1c Mutation: 32 strains or lines available

References

• Original: J:342939 Ferrari A, et al., Aster-dependent nonvesicular transport facilitates dietary cholesterol uptake. Science. 2023 Nov 10;382(6671):eadf0966
• All: 1 reference(s)