Egln3^tm1.1Jifan
Targeted Allele Detail

Summary

• Symbol: Egln3^tm1.1Jifan
• Name: egl-9 family hypoxia-inducible factor 3; targeted mutation 1.1, Jing Fang
• MGI ID: MGI:7624036
• Synonyms: PHD3-H196A^F
• Gene: Egln3 Location: Chr12:54225767-54250646 bp, - strand Genetic Position: Chr12, 22.9 cM
• Alliance: Egln3^tm1.1Jifan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:280720
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: A loxP site was inserted into intron 2 and a loxP2272 site, in inverted copy of exon 3 (where histidine codon 196 (CAT) was changed to alanine (GCT) (p.H196A)), a second loxP site, a second loxP2272 site, and an FRT site flanked neomycin resistance gene cassette, were inserted into intron 3. The neo cassette was removed through subsequent Cre-mediated recombination. This allele expresses the wildtype peptide. Only after Cre-mediated removal of the endogenous exon 3 and flipping of the mutated copy, will it express the mutated peptide. The mutation abolishes the prolyl hydroxylase activity of the encoded peptide. (J:280720)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Egln3 Mutation: 26 strains or lines available

References

• Original: J:280720 Xu Y, et al., Prolyl hydroxylase 3 stabilizes the p53 tumor suppressor by inhibiting the p53-MDM2 interaction in a hydroxylase-independent manner. J Biol Chem. 2019 Jun 21;294(25):9949-9958
• All: 2 reference(s)