Cers2^tm2.2Kwi
Targeted Allele Detail

Summary

• Symbol: Cers2^tm2.2Kwi
• Name: ceramide synthase 2; targeted mutation 2.2, Klaus Willecke
• MGI ID: MGI:7621229
• Synonyms: CerS2 Del, CerS2del79-120
• Gene: Cers2 Location: Chr3:95222102-95230910 bp, + strand Genetic Position: Chr3, 40.74 cM, cytoband F2
• Alliance: Cers2^tm2.2Kwi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:265853
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site, a copy of the wild type genomic sequence containing exons 3-11 including the stop codon and poly(A) signal sequences, an FRT site flanked neomycin resistance gene cassette and a second loxP site were inserted into intron 2. Codons 79P-120R in endogenous exons 3 and 4 were deleted and IRES sequences and the EGFP reporter gene were inserted into the 3' UTR between stop codon and poly(A) signal. Subsequent Cre-mediated recombination deleted wild type exons 3-11 and the neo cassette. This allele expresses the mutated peptide and the EGFP reporter. The deletion is located in the homeodomain of the encoded peptide. Transcription from this allele is 50% of WT and transcripts lack exons 3 and 4. Peptide expression is virtually absent. (J:265853)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cers2 Mutation: 60 strains or lines available

References

• Original: J:265853 Bickert A, et al., Inactivation of ceramide synthase 2 catalytic activity in mice affects transcription of genes involved in lipid metabolism and cell division. Biochim Biophys Acta Mol Cell Biol Lipids. 2018 Jul;1863(7):734-749
• All: 1 reference(s)