Srsf2^tm1.1Hko
Targeted Allele Detail

Summary

• Symbol: Srsf2^tm1.1Hko
• Name: serine and arginine-rich splicing factor 2; targeted mutation 1.1, Haruhiko Koseki
• MGI ID: MGI:7620430
• Synonyms: Srsf2^floxP95H
• Gene: Srsf2 Location: Chr11:116740727-116743920 bp, - strand Genetic Position: Chr11, 81.49 cM
• Alliance: Srsf2^tm1.1Hko page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:262169
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6 x 129/Sv

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A loxP and a VloxP site were inserted into the 5' UTR, a VloxP site, a lox2272 site, a copy of the lox site flanked portion of exon 1, in reverse orientation, where proline codon 95 (CCG) was changed to histidine (CAC) (c.284_285delCGinsAT:p.P95H), and an FRT site flanked neomycin resistance gene cassette, were inserted into intron 1. The neo cassette was removed through subsequent Flp-mediated recombination. This allele expresses wildtype peptides and only after Cre-mediated deletion of the endogenous exon 1 and flipping of the mutated duplicate, will it express the mutated peptide. (J:262169)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Srsf2 Mutation: 13 strains or lines available

References

• Original: J:262169 Kon A, et al., Physiological Srsf2 P95H expression causes impaired hematopoietic stem cell functions and aberrant RNA splicing in mice. Blood. 2018 Feb 8;131(6):621-635
• All: 1 reference(s)