Slc1a2^tm1.1Datr
Targeted Allele Detail

Summary

• Symbol: Slc1a2^tm1.1Datr
• Name: solute carrier family 1 (glial high affinity glutamate transporter), member 2; targeted mutation 1.1, Davide Trotti
• MGI ID: MGI:7620426
• Synonyms: EAAT2^CR, EAAT2-D504N
• Gene: Slc1a2 Location: Chr2:102489004-102621129 bp, + strand Genetic Position: Chr2, 54.13 cM
• Alliance: Slc1a2^tm1.1Datr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:262114
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Single point mutation
• Mutation details: Aspartic acid codon 504 (GAC) in exon 10 was changed to asparagine (AAC) (p.D504N) and an FRT site flanked neomycin resistance gene cassette was inserted into intron 10. The neo cassette was removed through subsequent Flp-mediated recombination. The mutation renders the encoded peptide resistant to cleavage by caspase 3. (J:262114)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc1a2 Mutation: 40 strains or lines available

References

• Original: J:262114 Rosenblum LT, et al., Mutation of the caspase-3 cleavage site in the astroglial glutamate transporter EAAT2 delays disease progression and extends lifespan in the SOD1-G93A mouse model of ALS. Exp Neurol. 2017 Jun;292:145-153
• All: 1 reference(s)