Igh^tm6.2Mbu
Targeted Allele Detail

Summary

• Symbol: Igh^tm6.2Mbu
• Name: immunoglobulin heavy chain complex; targeted mutation 6.2, Meinrad Busslinger
• MGI ID: MGI:7620246
• Synonyms: Igh⁸⁹⁰ⁱ
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:294166
• Parent Cell Line: A9 (ES Cell)
• Strain of Origin: (C57BL/6 x 129P2/OlaHsd)F1

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Inversion
• Mutation details: The allele was created by first inserting an Actb-neo cassette followed by a loxP (fl) site at position 116,237,220 (mm9, Chr.12) and blasticidin resistance gene (Bsd)-rox sequence into the middle of the VH gene cluster, followed by the insertion of a frt-Pgke promoter sequence-frt and a second lox (lox71) site (in the inverted orientation; ifl) at position 117,126,667 followed by Puro cassette in the Igh 5' region. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination inverted the floxed 890 bp. (J:294166)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 43 strains or lines available

References

• Original: J:294166 Hill L, et al., Wapl repression by Pax5 promotes V gene recombination by Igh loop extrusion. Nature. 2020 Aug;584(7819):142-147
• All: 1 reference(s)