Neurog3^tm1.2Annp
Targeted Allele Detail

Summary

• Symbol: Neurog3^tm1.2Annp
• Name: neurogenin 3; targeted mutation 1.2, Anna Philpott
• MGI ID: MGI:7620222
• Synonyms: WT Ngn3^eYFP
• Gene: Neurog3 Location: Chr10:61968869-61970542 bp, + strand Genetic Position: Chr10, 32.35 cM, cytoband B4
• Alliance: Neurog3^tm1.2Annp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241637
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: A loxP site was inserted into intron 1 and an FRT site flanked puromycin resistance gene cassette, a second loxP site, and a copy of exon 2 (with CHYSEL (cis-acting hydrolase element) sequence and the EYFP gene inserted in-frame at the 3' end of the CDS) were inserted downstream of the gene. The puro cassette was removed through subsequent Flp-mediated recombination. The floxed endogenous exon 2 was deleted through after Cre-mediated recombination, allowing expression of the reporter peptide in conjunction with the wildtype neurogenin 3 peptide from the exon 2 copy. (J:241637)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Neurog3 Mutation: 21 strains or lines available

References

• Original: J:241637 Azzarelli R, et al., Multi-site Neurogenin3 Phosphorylation Controls Pancreatic Endocrine Differentiation. Dev Cell. 2017 May 08;41(3):274-286.e5
• All: 1 reference(s)